How to prepare & standardization 0.1 N iodine solution

0.1 N Iodine Solution

0.1 N Iodine Solution 

 Preparation of 0.1 N Iodine Solution                   

  • Take 12.7 g of iodine and 60 g of potassium iodide (KI) to an 800-mL beaker, add 30 mL of water, and stir until solutions complete. 
  • Dilute with water to 500 ml, and filter through a sintered-glass filter. 
  • Wash the filter with about 15 ml of water, transfer the combined filtrate and washing to a 1-L volumetric flask, dilute to the mark with water, and mix. 
  • Store the solution in a glass-stoppered, amber-glass bottle in a cool place.

 Standardization of 0.1 N Iodine Solution                               

  • Transfer 1 g of primary standard arsenic trioxide (As2O3) to a platinum dish, and dry at 105°C for 1 h. Cool in a desiccator.
  • Weigh accurately 0.20 g of the dried As2O3 and transfer to a 500-ml conical flask.
  •  Add 10 ml of sodium hydroxide solution (NaOH, 40 g/L), and swirl to dissolve. When solution is complete, add 100 mL of water and 10 ml of sulfuric acid (H2SO4, 1 + 35), and mix. Slowly add sodium bicarbonate (NaHCO3) until efferves-cence ceases, 
  • Add 2 g of NaHCO3 in excess, and stir until dissolved. 
  • Add 2 ml of starch solution (10 g/L) and titrate with the iodine solution to the first permanent blue color.




*Warning—Arsenic trioxide is extremely toxic, avoid ingestion

 Calculation                       



Normality of the iodine solutions


I = w /0.04946*v
where:
I= normality of the iodine solution,
w= grams of As2O3 used, and
V= millilitres of iodine solution required for titration of the solution.


Ex
0.2202 = grams of As2O3 used, and
44.8 = millilitres of iodine solution required for titration of the solution.
 I = 0.2202/0.04946*44.8
   = 0.0993 N







 Stability                        



*Re-standardize sealed bottles monthly.
*Re-standardize open bottles weekly.



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