CONTROL OF WAVELENGTH
Holmium Oxide Solution
Weight accurately 1.0 gm of Holmium Oxide and dissolve it in 1.4 M Perchloric acid solution. Make up to 25 ml with the same solvent.
Go to spectrum mode
- Make sure that Reference and sample cell holder is empty and press button for baseline correction(200 to 700).
- Then keep the Holmium oxide filter in the sample cell holder
- Close the cell compartment
- Scan in the range of 200 to 700 nm for spectrum.
- After scanned (go to Operations–click Peak Pick)
- The spectrum shall show maximum at the following wavelength
- And record the observed maxima.
| Maxima at Wavelength (nm) | Limit (nm) |
|---|---|
| 241.15 | 240.15 to 242.15 |
| 287.15 | 286.15 to 288.15 |
| 361.5 | 360.5 to 362.5 |
| 536.3 | 533.3 to 539.3 |
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| CONTROL OF WAVELENGTH(UV calibration) |
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| CONTROL OF WAVELENGTH ( UV calibration ) |
More Topic related to Uv calibration
If calibration is satisfactory then affix the status label on it with the next due calibration date.
Frequency
Once in a 3 month